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Administrative Work |
Laboratory Work |
Human Practice |
| April 2016 |
Settlement of lab duties and poitions
Lab safety Training
Started regular meetings every week
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N/A |
N/A |
| May 2016 |
Recruitment of ideas and prepare preliminary data for selection. Project related to pancreatic cancer diagnosis by Toehold Switch is selected |
| June 2016 |
| July 2016 |
| August 2016 |
Cloning of switches and its
corresponding triggers into backbones with significant “ON/OFF”ratio in vivo.
Modified the switch by using new chromoprotein (amajLime and amilCP) by traditional cloning method to produce intuitive readout.
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Attended Tainan iGEM Conference |
| September 2016 |
Identified
problems to design Toehold Switch manually and outline programme functions for semi-automatic toehold switch design.
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Conducted exhibition and questionnaire related with synthetic biology in CUHK information day and reached secondary school students in Hong Kong. |
| October 2016 |
Reviewing the impact and necessity of technology in nowadays society, we decided to use Toehold Switch to subtype Influenza A. |
Request for sponsorship. |
| November 2016 |
Brainstorming and literature reviewing on how to maximize the“ON/OFF” ratio of Toehold switch. |
Constructed programs which embedded with Influenza A sequence database and Vienna RNA package.
Failed to test the system in cell free system due to insufficient materials and resources.
T7-rna-polymerase amplification system is cloning to yield higher “ON/OFF”ratio. |
| December 2016 |
Requested for sponsorship.
Skyped the Pakistan iGEM team to explore the potential of collaboration.
Continued fundraising.
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| January 2017 |
| February 2017 |
Organised day camp, “One Day Biochemist”, for secondary school students to try out working in standard laboratory. |
| March 2017 |
Completed individual registration for iGEM 2018 |
Successful cloning of standard backbone, which allows standardized toehold switch cloning with less false positive results.
Unsuccessful cloning of T7-rna-polymerase amplification system.
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Meeting with Joseph Sung, who is a medical expert and Vice Chancellor of CUHK. |
| April 2017 |
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Designed the product construction of Toehold Switch and outlined the prototype (user manual).
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| May 2017 |
Reviewed the updated metal requirement and revised the division of work |
Continued cloning Toehold switch for Influenza A subtyping.
Characterization on ability of fluorescent proteins by cloning amajLime and mRFP to pSB1A2 and pSB1C3 with constitutive promoter J23100 under different pH environment.
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| June 2017 |
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| July 2017 |
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Perform co-transformation to verify the "ON/OFF ratio: of new set of switch and trigger |
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| August 2017 |
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| September 2017 |
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Collaboration with UCCKE. Fine-tunning the protocol to optimised conditions. |
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| October 2017 |
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Draft the hardware model and kit for Dr. Switch. |
| November 2017 |
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