Team:MSU-Michigan/Current


Current from Induction

deltamtrB_GFPmtrB Induced V Uninduced

<figure><img src="T--MSU-Michigan--currentmtrB170725.png" width="366.5" height="300"></figure>

Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.

deltamtrB_GFPmtrB Induced V Uninduced

<figure><img src="T--MSU-Michigan--currentdeltamrtBGFPinduced.png" width="366.5" height="300"></figure>

The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.

Multiple Strains

<figure><img src="T--MSU-Michigan--current170714.png" width="366.5" height="300"> </figure>

Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.

Anaerobic Current

<figure><img src="T--MSU-Michigan--currentanaerobic20170911.png" width="366.5" height="300"></figure>

Anaerobic current showing how current induction can take the place of GFP induction as a marker for gene expression. IPTG induction occurs right at the small dip right around the 2 hour mark.