Wiki/Testing/protocolsmethods

• RNA Adsorption Test on first 3D print
• Protocol: Total RNA Purification with Norgen Kit
• Participants: Jorge
• Notes:
  • Total RNA sample 5 used, prepared on Friday 02/06 by Jorge (Concentration: 126 ng/µl)
  • Procedure:
    • Take 1 µl presample from stock ; add with 2 µl nf H2O and 3 µl 2x RNA LD
    • Put 5 µl of sample on device
    • Take 1 µl of sample immediately, add with 2 µl nf H2O and 3 µl 2x RNA LD
    • Wait 15 minutes
    • Take 1 µl of sample immediately, add with 2 µl nf H2O and 3 µl 2x RNA LD
    • Store at -80 °C
    • What still needs to be done:
      • Cook samples at 95 °C for 10 minutes and put directly on ice afterwards (to prevent refolding)
      • Load on Gel and quantify