Results

Microtubule

Plasmid construction

We have accomplished the construction of 6 main parts whose functions are described respectively in previous design page. (microtubule module)

figure 1

Protein expression

These are images recorded by the Fluorescence microscope.The engineered yeasts were induced at 30°C for 20 hours. And from the image, we can notice that our engineered yeasts have successfully expressed our recombinant proteins mCherry-αand mCherry. Moreover, the expression rate of mCherry is almost up to 100%.

figure 2 Induced 20h in SG-Ura medium；A,B: recipient strain with empty plasmid

The image shows the results of a Western blot analysis carried out with an anti-V5 antibody. The target proteins are expressed by the engineered yeasts which contain pYCα-alpha, pYCα-beta, pYCα-mCherry， pYCα-mCherry-αtubulin and pYD1-beta, respectively.