Influenza A can be spread rapidly throughout poultry flocks and cause severe illnesses, or even deaths in human. The most notorious pandemic was the "Spanish Flu" in 1918, which killed 50 million people worldwide (1). Influenza A virus poses large social and economic burden. Each year in the United States, it is estimated that around 600,000 lives and 90 billion US
dollars are lost due to influenza A virus (2). Influenza A can be subtyped according to the types of hemagglutinin (HA) and neuraminidase (NA) on the virus surface. Since there are 16 types of HA and 9 types of NA, there are 144 possible subtypes. Different Influenza A subtype show different properties. For example, the mortality rate of inflection by the subtypes H5N1 and H7N9 is much higher than that of H1N1.
Altogether, the project aims to apply toehold switch technology in influenza A subtyping and offer a convenient tool to facilitate the design of toehold switch. It is hoped that our method could suppress the spread of pathogenic Influenza in a timely manner.