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{{{toc}}} Engineered M13 modelling

Wet- and dry-lab back and forth:

Reste à rédiger, et ça je ne peux pas le faire seul

What are we modeling?

Reste à rédiger: décrire succintement le cycle de M13, idéalement avec un schéma (je peux m'en occuper. Du schéma aussi s'il n'y a pas de graphiste dispo)

First iteration of the model:

We started with an excessively simple model to see wether our understanding of our system was profound enough to allow room for such simplifications. We made the following assumptions in this spirit:

Table 1: assumptions of our "simplistic" model
Assumption	Reasonning
Neglecting cooperative effects
Constant rate of p5 transcription/traduction
One M13KO7 per factory cell
Phage assembly isn't a limiting factor
Neglecting stochasticity

Which led to the following model:
insérer image que j'ai dans la présentation que j'ai faite, mais en mieux dessinée / plus travaillée / classe / raccord avec le reste du thème graphique + comment je mets les images sur le site pour qu'elle s'intègrent dans le wiki? Est-ce que je les met sur internet sinon?
Simplistic model

Which yielded the following results:
là aussi mon graphique n'est pas adapté esthétiquement

Second iteration:

This time, we decided to adapt the model from Smeal et al. of a "wild-type" M13's biology to fit our own engineered version of M13 and better inform wetlab experiments, as well as explain some of their preliminary findings!
Model validation: compare particle profile to that obtained in wetlab
--> Insufficient p3 could cause the packing of several DNA molecules per phage.
What is the ideal number of plasmid copies if we produce p3 on a different plasmid?

Bibliography: