Material&Methods
- 1.Parts
- 2.Primer list
- 3.Materials
- 3-1. Kit
- 3-2. Restricton Enzyme
- 3-3. Polymerase
- 3-4. DNA ligase
- 3-5. Marker
- 3-6. Organism
- 3-7. Antibiotics
- 3-8. Equipment
- 3-9. Backborns
- 3-10. Buffer
- 3-11. SDSPAGE/Western blotting
- 4.Methods
- 4-1. Miniprep
- 4-2. Gel Extraction
- 4-3. Restrict Enzyme Digestion
- 4-4. Ligation
- 4-5. Transformation
- 4-6. PCR
- 4-7. Sequencing
- 4-8. RT-PCR
- 4-10. Western blotting(Membrane fraction)
1.Parts
2.Primer list
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3.Materials
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