Team:INSA-UPS France/Parts
BBa_J04450 was tested in the Vibrio harveyi background. BBa_J04450 biobrick was cloned in a broad host range plasmid (pBBR1MCS-4) and conjugated into Vibrio harveyi to demonstrate the production of RFP in this chassis.
To learn more: <partinfo>BBa_J04450</partinfo>, see our results
The pGAP promoter was used as a constitutive promoter and as a homology region to make easier the genomic recombination of our synthetic construction in Pichia pastoris genome.
The promoter was characterized by RT-qPCR using d-ny15 as reporter gene.
To learn more: <partinfo>BBa_K431009</partinfo> , see our results
The α-factor sequence contains a kozak region and a signal sequence to secrete the produced peptides. The functionality of the signal factor was investigated by demonstrating that AMP are present in the supernatant through a toxicity assay.
To learn more: <partinfo>BBa_K1800001</partinfo>, see our results
This part contains the first half of Vibrio harveyi quorum CqsS receptor which is able to detect CAI-1 family molecule. The receptor is driven by a constitutive promoter.
This part has been designed due to IDT gBlocks synthesis requirements. The ordered gBlocks size should not exceed 3kb (the total length of the Vibrio harveyi circuit is 6929 bp). The XhoI restriction site naturally present in the gene was used reassemble the cqsS* sequence. Therefore, it was impossible to use pSB1C3 vector because of the presence of a XhoI restriction on the vector. This part was then subcloned in pBR322.
iGEM UPS-INSA Toulouse 2017
Parts
New parts submitted to the registry
Name
Function
Type
Part
State
<partinfo>BBa_K2278001</partinfo>
QS molecule generator
basic
working
<partinfo>BBa_K2278002</partinfo>
QS molecule generator
basic
not released
<partinfo>BBa_K2278011</partinfo>
Diacetyl generator
basic
issues
<partinfo>BBa_K2278021</partinfo>
D-NY15 AMP generator
basic
Working
<partinfo>BBa_K2278022</partinfo>
Leucrocin I AMP generator
basic
unsuccessful
<partinfo>BBa_K2278023</partinfo>
coT2 AMP generator
basic
unsuccessful
Existing Parts we have contributed to characterized
<partinfo>BBa_J04450</partinfo>: RFP coding device
<partinfo>BBa_K431009</partinfo>: glyceraldehyde 3-phosphate dehydrogenase promoter (pGAP)
<partinfo>BBa_K1800001</partinfo>: alpha factor secretion signal
Parts used in our project but not submitted to the registry
Vh1: first part of Vibrio harveyi gene circuit
