Team:Chalmers-Gothenburg/Results
Overview
The lung cancer biosensor was divided into two major parts: receptor integration and pathway. The native membrane localized GPCRs STE2 and STE3 were substituted with the two heterologous GPCRs Olf1258 and Ri7 in two different yeast mating types. These two heterologous GPCRs were fused with GFP to test membrane localization. Finally, the functionality of the GPCRs was tested using the plasmid pGFP, were pFUS1 is coupled to GFP. pFUS1 is a mating gene promoter induced after GPCR induction. For testing the functional pathway, the loxP-pTEF-loxP switch was investigated. Before the pTEF promoter is turned, GFP is expressed. Therefore, the decrease in GFP expression was tracked after the turning of the promoter, indicating that the promoter switch was successful.
Receptors
Integration of Olfr1258 and Ri7
For the biosensor to work, modifications of the two yeast strains, a and α, needed to be performed. One major modification was the substitution of the native GPCRs, Ste2 and Ste3, with the two heterogenous olfactory receptors, Ri7 and Olfr1258. This was executed using a CRISPR system with Cas9 and gRNA specific to STE2 and STE3, for more information see Project constructs.
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Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Sed consequat mattis interdum. Aliquam et velit fermentum velit imperdiet rhoncus. Donec accumsan molestie ornare. Duis eu odio in tortor ullamcorper aliquam. Vivamus molestie fermentum urna, vitae commodo mauris consequat in. Curabitur porttitor pulvinar purus, ut varius nibh tempus id. Nulla dictum lectus ut erat condimentum, sit amet euismod nibh eleifend. Maecenas molestie efficitur urna vel semper. Nullam in turpis eleifend, venenatis ex tincidunt, tristique sapien. Suspendisse eget facilisis dolor. Integer congue nisi eu magna consectetur, a bibendum nibh accumsan. Vivamus fermentum metus id lectus porttitor congue. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Nulla metus quam, dapibus ac volutpat ut, dapibus sit amet felis.
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Sed consequat mattis interdum. Aliquam et velit fermentum velit imperdiet rhoncus. Donec accumsan molestie ornare. Duis eu odio in tortor ullamcorper aliquam. Vivamus molestie fermentum urna, vitae commodo mauris consequat in. Curabitur porttitor pulvinar purus, ut varius nibh tempus id. Nulla dictum lectus ut erat condimentum, sit amet euismod nibh eleifend. Maecenas molestie efficitur urna vel semper. Nullam in turpis eleifend, venenatis ex tincidunt, tristique sapien. Suspendisse eget facilisis dolor. Integer congue nisi eu magna consectetur, a bibendum nibh accumsan. Vivamus fermentum metus id lectus porttitor congue. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Nulla metus quam, dapibus ac volutpat ut, dapibus sit amet felis.
Title 2
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Sed consequat mattis interdum. Aliquam et velit fermentum velit imperdiet rhoncus. Donec accumsan molestie ornare. Duis eu odio in tortor ullamcorper aliquam. Vivamus molestie fermentum urna, vitae commodo mauris consequat in. Curabitur porttitor pulvinar purus, ut varius nibh tempus id. Nulla dictum lectus ut erat condimentum, sit amet euismod nibh eleifend. Maecenas molestie efficitur urna vel semper. Nullam in turpis eleifend, venenatis ex tincidunt, tristique sapien. Suspendisse eget facilisis dolor. Integer congue nisi eu magna consectetur, a bibendum nibh accumsan. Vivamus fermentum metus id lectus porttitor congue. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Nulla metus quam, dapibus ac volutpat ut, dapibus sit amet felis.
Subtitle 1
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Sed consequat mattis interdum. Aliquam et velit fermentum velit imperdiet rhoncus. Donec accumsan molestie ornare. Duis eu odio in tortor ullamcorper aliquam. Vivamus molestie fermentum urna, vitae commodo mauris consequat in. Curabitur porttitor pulvinar purus, ut varius nibh tempus id. Nulla dictum lectus ut erat condimentum, sit amet euismod nibh eleifend. Maecenas molestie efficitur urna vel semper. Nullam in turpis eleifend, venenatis ex tincidunt, tristique sapien. Suspendisse eget facilisis dolor. Integer congue nisi eu magna consectetur, a bibendum nibh accumsan. Vivamus fermentum metus id lectus porttitor congue. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Nulla metus quam, dapibus ac volutpat ut, dapibus sit amet felis.
