Team:Calgary/Secretion
Secretion
Overview
After the E. coli BL21 (DE3) engineered by the Synthesis subgroup has produced polyhydroxybutyrate (PHB), it is stored as intracellular granules that range in size from 60-80nm. This creates a challenging problem: efficient extraction of the desired PHB from inside the cell. During the early stages of our project, we explored many possible PHB extraction methods.
Type 1 Secretion
Our Secretion System
Our Construct
Experiments
Future Directions
Works Cited
