Protocols

General Protocols

Chemical Transformation

Colony PCR

Consumables

Cryostock of Bacteria

Electroporation

Gel Extraction

Gibson Assembly

Ligase Chain Reaction

Miniprep

PCR Clean-Up

Phusion PCR

Preparation of Chemically Competent Cells

Preparation of Electrocompetent Cells

Restriction Ligation

Fluorescence Measurement to Obtain the AHL Dose-Response Curve

SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin

Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle