Team:TokyoTech/Demonstrate

To realize the system of "Coli Sapiens" and check the feasibility, we constructed a series of mathematical models and simulated it comprehensively based on test results and characterized parts.

This simulation provided suggestive evidence for the improvement of wet-lab experiments. In iGEM community, so far, a number of studies have demonstrated information processing among bacteria. This year, however, we added human cells which are obviously more complex from bacteria.

By co-culturing them, we tried the information processing between two types of cell clusters which significantly differ in growth rate and production rate. In addition, we selected important elements in the complex system, abstracted models and then simulated it. As a result, it turned out that human cells can controll the population of E. coli and the necessary conditions for codependent relationship.

Through this project, we succeeded in engineering a new living system which bacteria and human cells can co-exist. We believe this project will represent a step on the way to a whole new dimension of iGEM research.