Team:Munich/Protocols

-_- Project Description Design Demonstrate Measurement Applied Design Final Results Achievements Wetlab Protocols Notebook Safety Parts Improved Part Part Collection InterLab Model/Software Model Software Collaboration Hardware Detector Quake Valve Paperstrips Sample Processing Human Practices Silver Gold Engagement Collaborations Team Team members Sponsors Attributions Gallery

Protocols According to all known laws of aviation, there is no way a bee should be able to fly. Its wings are too small to get its fat little body off the ground. The bee, of course, flies anyway because bees don't care what humans think is impossible. Yellow, black. Yellow, black. Yellow, black. Yellow, black. General Cas13a Read-out Target Other Agarose gel electrophoresis PCR Miniprep Restriction digest Ligation Glycerol stock Protein purification with Ni-NTA Protein purification with Akta SDS page RNAse activity protocol Lyophilization Protein expression Phenol-chlorophorm purification of RNA Phenol-chlorophorm purification of DNA Urea gel for RNA detection In vitro transcription RNA purification with Norgen Kit FINA method for RNA extraction FINA method for DNA extraction First strand cDNA syntesis (NEB) RPA RPA on paper RPA TX Chem trafo Electro trafo Chemically competent cells Electro-competent cells Dephosphorylation Golden Gate Gibson PCR cleanup Sequencing sample prep Soft litography AuNP preparation AuNP-DNA conjugation AuNP linkage AuNP cleavage Primers and sequences