Biobrick Tutorial 2017

On Friday of the second weekend of March (9th-11.th of march), DTU BioBuilders stood in anticipation for the first arrival of the invited Nordic iGEM teams

Teams from Norway: Universitetet i Oslo, UiO, Sweden: Chalmers University of Technology , and Denmark: University of Copenhagen, (KU) and University of Southern Denmark, (SDU) marked their presence. Counting 44 people in all made this year’s biobrick tutorial the most attended as of yet.

The purpose of the Biobrick tutorial is to demonstrate the modularity and versatility of the Biobrick beyond on a theoretical level, to socialize and initiate a foundation for further collaboration and to lower the distance between the registry and the members of the respective teams - from teams to start exploring the registry. And to get a clearer idea of the concepts of “Digestion”, “Ligation”, “transformation” and “plate spreading” keeping in mind that the members of the teams have different academic backgrounds and are enrolled in various programs.

Thus, the bulk of the weekend (Saturday) was spent getting hand-on experience with the 3A assembly. This was achieved in the form of a laboratory exercise, where different genetical parts were combined by small teams of four. We achieved this by combining the BioBrick plasmid backbone (pSB1C3) with a purple-blue chromoproteins (RBS-amilCP) under control of a constitutive promoter.