The goal of this project is to engineer a biological circuit utilizing multiple e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic. Once this construct is designed and constructed the circuit will be tested to demonstrate is viability using a cell free lateral flow paper based system deigned to detect Arsenic.
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Throughout this project we worked closely with several local non-profits in the Tacoma urban area. The goal of the project was to design a successful biological circuit utilizing e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic+.
After the circuit was designed and constructed the next step was to demonstrate the circuit as viable in a cell of Arsenic. To accomplish this goal we worked with Urban Waters, Readiness Acceleration and Innovation Networking (RAIN),and Charles Wright Academy.
By utilizing community involvement we hope to both increase pre-collegiate interest in STEM related fields as well as increase the communal understanding of the environment and provide a inexpensive and effective tool for the detection of potentially toxic metals.
Synthetic Biology
What it is and How it Played a Role in Our Project
Synthetic Biology is the shift from understanding and modifying biology to treating biology as an engineering discipline where basic building blocks are assembled from the ground up into larger engineering systems.
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