Results
In the part of lab work, we have designed three biosensor sequence and improved an old part, J23000. What's more, all the three design have been demonstrate by us.
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Figure 3. Influence of Formaldehyde Induce Time on Fluorescence Expression |
Figure4.A photograph of E.coli cells containing the formaldehyde-induced RFP expression plasmid, or without formaldehyde induction, and re-suspended in PBS buffer(pH7.4) |
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It is worth to be mentioned that the team OUC help us demonstrate the result. |
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In the experiment, we proved that the sequence worked well and was useful to detect hydrogen sulfide | |
Figure1.Whole-cell sequence dual-enzyme digestion |
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Figure 2.a)RFP responsiveness of the detector system. |
There is a composite of hydrogen sensor full length sequence. The order of the elements is: HoxA-HoxB-HoxC-HoxJ-terminator-HoxP-EGFP. The sequence of HoxABCJP comes from Ralstonia eutropha H16 megaplasmid pHG1. The hole sequence acts as an hydrogen sensor. When the amount of hydrogen goes to a higher level, Fluorescence intensity increases apparently. | |
The sequence was a good detecter in the lab work. | |
Figure1. Coomassie Brilliant Blue R-250-stained SDS-Page analysis of recombinant E.coli expressing hoxABCJ-terminator-hoxp-gfp |
Fingure 2. Western blot analysis of recombinant E.coli expressing his-hoxA |
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Figure 3. Influence of H2 concentration on fluorescence expression |