Team:Baltimore Bio-Crew/Team
Advisors
Sarah Laun
We are evaluating our parts by verifying that they can be used to degrade PET plastic. We are setting up an experiment in which we will test for the the ability of E.coli bacteria engineered with each part to degrade PET plastic. Glass culture tubes were set up containing LB media and small squares of PET plastic that had been weighed beforehand. The approximate dimensions of the plastic squares were 20x15x0.5mm, and they weighed between 101 and 89 mg. After the bacteria with the gene have been added, the plastic will be weighed twice a week to check for lost material. Some tubes will be incubated at 30 degrees Celsius because that is the best growth temperature for the Ideonealla bacteria, and some will be incubated at 37 degrees Celsius because that is the best temperature for E. coli.
Lisa Schifele
We are evaluating our parts by verifying that they can be used to degrade PET plastic. We are setting up an experiment in which we will test for the the ability of E.coli bacteria engineered with each part to degrade PET plastic. Glass culture tubes were set up containing LB media and small squares of PET plastic that had been weighed beforehand. The approximate dimensions of the plastic squares were 20x15x0.5mm, and they weighed between 101 and 89 mg. After the bacteria with the gene have been added, the plastic will be weighed twice a week to check for lost material. Some tubes will be incubated at 30 degrees Celsius because that is the best growth temperature for the Ideonealla bacteria, and some will be incubated at 37 degrees Celsius because that is the best temperature for E. coli.
Students
Ella Coleman
We are evaluating our parts by verifying that they can be used to degrade PET plastic. We are setting up an experiment in which we will test for the ability of E.coli bacteria engineered with each part to degrade PET plastic. Glass culture tubes were set up containing LB media and small squares of PET plastic that had been weighed beforehand. The approximate dimensions of the plastic squares were 20x15x0.5mm, and they weighed between 101 and 89 mg. After the bacteria with the gene have been added, the plastic will be weighed twice a week to check for lost material. Some tubes will be incubated at 30 degrees Celsius because that is the best growth temperature for the Ideonealla bacteria, and some will be incubated at 37 degrees Celsius because that is the best temperature for E. coli.
Naige Correal-Winters
Hi, my name is Naige Correal-Winters. I am a junior at Centennial High School. I am not quite sure what I want to do in the future. I joined the Baltimore Biocrew after finding BUGSS while working on a project with CRISPR, and I learned about the team and what they are doing to help Baltimore harbor.
Aleem Prince
Sawadee Kha, my name is Aleem Prince. I am a senior at Patterson High school. In the future, I would like to become a computer programmer or a game designer. I visited BUGSS on a field trip with Building STEPS and I saw a presentation of the Baltimore Biocrew’s project. The project was focused on the Inner Harbor - the first place I visited when I came to America - hence, I decided to join. I wasn’t a big fan of biology. However, I want many people to enjoy and make memories at the Inner Harbor as I did.
Zion Smith
Hey, my name is Zion Smith and I am a Junior at Baltimore City College High School. I would like to pursue Pre-medical studies in college and hopefully move on to medical school and become an acute care surgeon. I love science, health, and the environment and I am constantly looking for ways to contribute to my passions. BUGSS is a place where many issues within the environment and the health of Baltimore can be tackled. I joined the Baltimore Bio-Crew not only because I enjoy biology, but also because of the great work that is being done in the lab. I hope to continue working on ways to impact the environment of my city in a positive way!
Shatera McNair
Hello, my name is Shatera McNair, and I’m a full IB senior at The Baltimore City College. I aspire to be a family physician and to work in public health, but The Baltimore Biocrew’s project last year inspired me to join and explore the unfamiliar research aspect of health with the goal of eliminating the environmental negativity in the heart of our city. I never imagined I would have the privilege to have such a powerful and direct impact on my community while using the materials I learned in science and in the lab!
Mercedes Ferandes
My name is Mercedes Ferandes and I am a junior at Bard High School Early College. I have aspirations of being a physician in the future, as well as advocating for those who face health disparities in my community. I joined the Baltimore BioCrew because I saw the harmful effects that plastic had on the citizens of Baltimore and knew that something should be done about it.
Mercedes Thompson
We are evaluating our parts by verifying that they can be used to degrade PET plastic. We are setting up an experiment in which we will test for the ability of E.coli bacteria engineered with each part to degrade PET plastic. Glass culture tubes were set up containing LB media and small squares of PET plastic that had been weighed beforehand. The approximate dimensions of the plastic squares were 20x15x0.5mm, and they weighed between 101 and 89 mg. After the bacteria with the gene have been added, the plastic will be weighed twice a week to check for lost material. Some tubes will be incubated at 30 degrees Celsius because that is the best growth temperature for the Ideonealla bacteria, and some will be incubated at 37 degrees Celsius because that is the best temperature for E. coli.
