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We envision a portable,fully automated, fluidic device that can process biological sample in the field. We need to consider that every part of our device that gets in contact with our sample needs to be disposable and replaceable. Therefore we designed an replaceable fluidic chip that is controlled via an external device We want to use electrically controlled quake valves to precisely move fluids. We therefore need an supply of high pressure which can be providet by an bicycle wheel.
To control fluid flow in our final device we constructed pneumatic controlled water valves. We use electric powered air valves to control these so-called quake valves and build a circuit to control the air valves with a microcontroller. This enables us to precisely move fluids on our final device via a software interface. We constructed macroscopic quake valves with 3D printed negatives out of PDMS via soft lithography. The valves can be scaled down and are require now special equipment for their manufacture. A detailed protocol for manufacturing macroscopic fluidic chips with 3D Printed negatives can be found here at the subitem "Soft lithography".
The valve is made of three PDMS layers. The lower layer carries the water channel with a sinus shaped dome at the valve position. The middle layer is just a thin and therefore elastic PDMS film. The upper layer carries the air channel which as a cylindric air reservoir at the valve position. The three layers are shown in the explosive drawing below.
Explosive drawing of a quake valve.
If air pressure is applied to the upper channel the middle layer will be pressed in the sinus shaped dome of the upper channel and block the water channel. We chose a sinus shape for the lower channel to enable a smooth contact between the thin layer and lower layer. This mechanism is illustrated in the figure below
Function of a quake valve.
The Air channel is connected via tubes to two normally closed electric air valves. One to release pressure for opening the water valve and one to apply new pressure for closing the water valve.
We need a control circuit for the air valves because they are powered with high currents and consist of an electric coil that causes voltage pikes which can be harmful to a microcontroller
The electric valve is connected to the drain of drain of a N Channel MOSFET. The drain is connected via a voltage divider to a digital pin. When the digital Pin is set to 5V, the voltage at the gate is above the threshold voltage, current flows through the Valve and the air channel is opened. A diode in blocking direction parallel to valve damps the voltage spike caused by turning of the valve. The circuit is shown in the figure below.
Control circuit for the electric valves.
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