Team:Munich/Protocols

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Protocols For our project we used different methods which are described in respective protocols. We also included a detailed list of all chemicals, materials and the aim of each experiment. This page is for everybody who wants to reproduce our work or wants to get an overview, but also to share our experiences with the iGEM community. General Cloning Cas13a Readout Other Agarose gel electrophoresis PCR Minipreparation Restriction digest Ligation Glycerol stock Ni-NTA agarose protein purification Äkta protein purification SDS PAGE RNase activity Lyophilization Expression of Cas13a and TEV Protease Phenol/Chlorophorm purification of RNA Phenol/Chlorophorm purification of genomic DNA Denaturing Urea PAGE In vitro Transcription RNA purification with Norgen Kit FINA method for RNA extraction FINA method for DNA extraction First strand cDNA syntesis RPA RPA on paper RPA TX Chemical transformation Electro transformation Chemically competent cells Electro competent cells Dephosphorylation Golden Gate Assembly Gibson Assembly PCR clean-up Sequencing sample preparation Soft litography AuNP preparation AuNP-DNA conjugation AuNP linkage AuNP cleavage Protein and nucleic acid sequences Proteins Nucleic acids