Team:Lambert GA/InterLab


Interlab Study



Introduction




Data


The LUDOX Solution provided to our team was stored in a freezer for an extended period of time, eventually rendering in dysfunctional. The fluorescein solution also appeared to not express color as expected. Due to this, we utilized the LUDOX solution and fluorescein provided to the UGA team in order to obtain our OD Reference and fluorescein standard curves. The transformations were conducted at Lambert High School, plated and parafilmed, and transported to UGA for inoculation. During sample measurement, we obtained several OVRFLW errors in certain samples, and despite changing gain settings, the error was still registered. We were unable to obtain data for those replicates, but did obtain data for the others. We utilized UGA’s Biotek Synergy H4 Plate Reader for our measurements, and conducted all measurements under the supervision of the UGA iGEM Team and their advisors.

Excel Spreadsheets with Graphs

The spreadsheets with raw data are embedded below. Under the tab Fluorescein Standard Curve, there are the graphs created from our data.