Notebook.
All protocols we used for the methods can be found here:
Protocol
Week 1 (03/07-07/07)
• Prepared growth media
• Interlab Challenge 2017: for more information, please visit our Interlab site.
• E. Coli DH10B cells made chemically competent
• Golden Gate Assembly of BB1_01, BB1_02, BB1_03, BB1_04, BB1_05, BB1_06, BB1_07, BB1_08
Week 2 (10/07-14/07)
• PCR: 1, 2, 3, 4, 6, 15, 18, 20, 23, 24, 25, 26
Each PCR was loaded on a preparative gel and cut out before undergoing PCR-purification.
• Golden Gate Assembly: BB1_09, BB1_10, BB1_11, BB1_12, BB1_13, BB2_01(unsuccessful), BB2_02, BB2_03, BB2_04, BB2_05 (unsuccessful)
GG-products are then transformed into competent cells. Colonies with specific antibiotic resistances were selected out by using antibiotic-containing media. The next day, positives clones were confirmed via a OneTaq colony PCR and a gel electrophoresis before a Miniprep was made.
• pSIM5 pre-cultures (DH5alpha + BL21(DE3))
• Transformation of HSM174 + pSIM5 (unsuccessful)
• Yeast S288C gDNA extracted
Week 3 (17/07-21/07)
Everything we did in the third week...
Week 4 (24/07-28/07)
Everything we did in the fourth week...
Week 5 (31/07-04/08)
Everything we did in the fifth week...
Week 6 (07/08-11/08)
Everything we did in the sixth week...
Week 7 (14/08-18/08)
Everything we did in the seventh week...
Week 8 (21/08-25/08)
Everything we did in the eighth week...
Week 9 (28/08-01/09)
Everything we did in the ninth week...
Week 10 (04/09-08/09)
Everything we did in the tenth week...
Week 11 (11/09-15/09)
Everything we did in the eleventh week...
Week 12 (18/09-22/09)
Everything we did in the twelfth week...
Week 13 (25/09-29/09)
Everything we did in the thirteenth week...
