Team:Cadets2Vets/Demonstrate
GOAL OF OUR PROJECT
Our circuit was designed to utilize specific parts so that when the E. coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.
Demonstrate the Circuit
Before running the actual circuit, we ran control experiments to determine whether or not the GFP would indeed function as a reporter, meaning it is expressed by the plasmid.
The first control experiment drove GFP using a constitutive promoter: meaning that the GFP would always be produced. This was essentially only used to ensure that the GFP works in the paper ticket.
The second control experiment was regulated, meaning that GFP expression was dependent on a trigger. This tested if the GFP could be controlled/ regulated.
These experiments were performed using a commercial in vitro transcription/translation kit by Promega.
The results from both of these experiments are shown in the pictures below.
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