CCA iGEM is proud to participate in the 2017 iGEM interlab for the first time, and we’re happy to share our results with the community at large. The objective of this year’s interlab was to use an identical protocol for eight different plasmids (6 test devices, a positive, and a negative control) in expressing GFP and measuring it in common units to compare results across the world and across a variety of plate readers. This year, a new bicistronic design element (J364100) was used in an attempt to improve the accuracy and reliability of results worldwide in conjunction with the three promoters that were tested last year: J23101, J23106, and J23117. The new BCD was measured against the B0034 single ribosome binding site with the same promoters and against both a positive and negative control in I20270 and R0040. These plasmids were transformed on the pSB1C3 vector in standard fashion and tested to produce the results below.
Methodology
First we standardized our measurements in both LUDOX-HS40 and water by taking a set of measurements in a spectrophotometer and calculating a correction factor to convert our data into the standardized units that iGEM requested (OD600).
