Team:XMU-China/Contribution
The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the coral Acropora millepora, amilCP. In this year, we want to verify the characteristic of BFP.
-----* Experiment *-----Firstly, we got the biobrick called K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grown on the plate.
Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.
Xiamen University, Fujian, China
No. 422, Siming South Road, Xiamen, Fujian, P. R. China 361005
