Team:Exeter/Composite Part
Composite Parts
FimH sfGFP
We decided to use four different promoters to drive expression of our pili proteins. The T7 promoter (BBa_I712074) was chosen as it is a strong, well characterised promoter but as this could only be used in BL21(DE3) it was only used for initial expression studies. Two inducible promoters were chosen to allow control over expression in non pili-producing strains of E. coli: the rhamnose inducible promoter P_Rha (BBa_K902065) and the arabinose inducible promoter P_ara (BBa_I13453). Finally the constitutive promoter P_J23100 (BBa_J23100) was chosen.
| Name | Description | Base Pairs |
|---|---|---|
| BBa_K2324007 | pRha_FimH_22_sfGFP | 2020 |
| BBa_K2324006 | pRha_FimH_225_sfGFP | 2020 |
| BBa_K2324008 | pRha_FimH_258_sfGFP | 2020 |
FimH Fusion Proteins
| Name | Description | Base Pairs |
|---|---|---|
| BBa_K2324002 | pRha_FimH_22_His | 1102 |
| BBa_K2324001 | pRha_FimH_22_SynMT | 1474 |
| BBa_K2324003 | pRha_FimH_22_MouseMT | 1489 |
Fim Operon
| Name | Description | Base Pairs |
|---|---|---|
| BBa_K2324012 | pJ23100_Fim_Operon | 5944 |
| BBa_K1850013 | pAra_Fim_Operon | 5874 |
