Team:XMU-China/Contribution
We also improved the characterization of two existing BioBrick Parts although we've participated in the InterLab Measurement Study. The fluorescent protein is used widly for a reporter,such as GFP and RFP. To enrich the kinds of fluorescent protein, the iGEM11_Uppsala-Sweden supported some other colour proteins like BFP and YFP which are from the coral Acropora millepora, amilCP. In this year, we want to verify the characteristic of BFP.
-----* Experiment *-----Firstly, we got BBa_K1357009 from the 2017 DNA Distribution Kit Plates and transformed it to the E.Coli DH5α. After a one-week incubation, a big bacterial colony grown on the plate.
Secondly, we extracted plasmid from the bacteria, digested with XbaI and PstI, cloned it into plasmid BBa_J61002 with the promoter J23100, then transformed into E.coli DH5α again. We observed the strong blue just one day later. The result shows that BBa_K1357009 works well.
Xiamen University, Fujian, China
No. 422, Siming South Road, Xiamen, Fujian, P. R. China 361005
