Team:CCA San Diego/Improve
Improvement
We have submitted this part as a plasmid backbone
RK2 Broad Host Range Vector with Kanamycin Resistance
The goal of this improvement was to replace p15a origin of replication in pSB3K3 (source) with the RK2 ori to generate a shuttle vector as a broad host range vector (i.e. can replicate in E.coli and other microorganisms).
The RK2 origin of replication is a broad-host-range plasmid belonging to the incP incompatibility group that can be maintained in a large number of bacteria. The minimal region for replication and maintenance consists of an origin of replication, oriV, and the plasmid replication initiator protein TrfA protein, that activates oriV. The copy number of RK2 is about 4-7 per cell in E. coli, 3 in Pseudomonas aeruginosa, and 4-7 in Agrobacterium.
Canyon Crest Academy iGEM 2017 CC; |
