| 2017.04.01 |
HP |
Communicated with one of the authors of the article "Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation" online to get some latest progress. |
| 2017.04.08 |
Attempts in E.coli |
Got the Nitratireductor pacificus which contain our gene rdhANP. |
| 2017.04.10 |
Attempts in E.coli |
Started to culture N. pacificus. |
| 2017.04.11 |
Attempts in E.coli |
Extracted whole genome of N. pacificus. |
| 2017.04.12 |
Attempts in E.coli |
Got our gene rdhANP by PCR |
| 2017.04.21 |
Attempts in Bacillus subtilis |
Amplified rdhANP by colony PCR of N. pacificus |
| 2017.04.25 |
Preparation of the protoplast for Bacillus megaterium |
Made protoplast of B.megaterium |
| 2017.04.26 |
Attempts in B. subtilis |
Amplified rdhANP by colony PCR of N. pacificus |
| 2017.04.26 |
Preparation of the protoplast for B.megaterium |
Prepared prot-medium |
| 2017.04.27 |
Attempts in B. subtilis |
Agarose gel electrophoresis confirmed that the gene rdhANP is successfully amplified |
| 2017.04.27 |
Preparation of the protoplast for B.megaterium |
Prepared hyp-medium |
| 2017.04.29 |
Attempts in E.coli |
Amplified rdhANP by colony PCR of N. pacificus |
| 2017.04.30 |
Attempts in E.coli |
Cultured the E.coli with plasmid pET28a+ |
