Team:Calgary/MedalRequirements

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Judging Medal Requirements

BRONZE MEDAL

Register and Attend

The team has registered and will be attending the 2017 Giant Jamboree Boston from November 9th-13th!

Deliverables

• Wiki
• Poster
• Presentation
• Safety Forms
• Judging Form
• Registry Part Pages
• Sample Submissions

Attributions

Check out our Attributions page for detailed information concerning the contributions of team members, mentees, and advisors!

Characterization or Contribution

Our InterLab page shows how our team participated in the InterLab study this year!

SILVER MEDAL

Validate a Part

BBa_K2260001

We submitted a new gene to the registry to assist in the production of PHB: the phaJ gene that codes for PHB enoyl-CoA hydratase. part contains both the phaJ and phaC genes and produce PHB on its own in E. coli under the control of an IPTG-inducible promoter. HPLC was used to identify and quantify the PHB produced. Results can be found on our part's Registry page.

Collaboration

View our Canadian iGEM newsletter and wet-lab work with other iGEM teams on our Collaborations page!

Human Practices Silver

We carefully weighed the effects our project could have in a variety of applications, including possible benefits, challenges, and safety considerations . See our Human Practices Silver page for more info!

GOLD MEDAL

Integrated Human Practices

We continuously engaged in conversation with end-users, stakeholders, and experts in the field to inform the design and safety of our project. Find out more about our Integrated Human Practices!

Modelling

Our team worked on two types of mathematical modelling: kinetic and flux balance. You can check out the details of that on our Modelling page.

Improve a Previous Part

BBa_K2260000 and BBa_K2260003

We improved two parts. The first, BBa_K226000, was an improvement on Imperial College's phaCAB operon submitted in 2013. The gene order was changed to improve PHB yield (), codons were optimized for expression in E. coli, and restriction sites were removed to allow part compatibility in all iGEM RFC assembly standards. The second, BBa_K226003, was an improvement on SDU Denmark's phasin-HlyA part submitted in 2016. Codons were optimized for expression in E. coli.