Team:ETH Zurich/Parts
Parts
Overview
We submitted in total 14 new BioBricks to the iGEM registry! You can find all parts and their design below.
Basic Parts
| Part | Description | BioBrick |
|---|---|---|
| Bacterioferritin | Heme-deletion mutant of bacterial iron storage protein functioning as an MRI contrast agent | BBa_K2500000 |
| Azurin | Mature cytotoxic copper protein | BBa_K2500001 |
| p28 | Effector domain of azurin | BBa_K2500002 |
| pTlpA | Temperature-responsive promoter TlpA optimized for slight activation above 37°C and full activation at 45 °C | BBa_K2500003 |
| TlpA | Protein reversibly inhibiting the pTlpA promoter at temperatures below 37 °C | BBa_K2500004 |
| RBS_TlpA | Protein reversibly inhibiting the pTlpA promoter at temperatures below 37 °C and synthetic RBS | BBa_K2500005 |
| Protein E | Bacterial cell lysing protein E | BBa_K2500006 |
| RBSeng_TlpA | Bacterial cell lysing protein E | BBa_K2500007 |
| pTlpA_ProteinE | Bacterial cell lysing protein E regulated by heat-inducible promoter pTlpA and synthetic RBS | BBa_K2500009 |
| AND gate A | Synthetic promoter responsive to LldR and luxR | BBa_K2500010 |
| Best Basic Part: AND gate B |
Synthetic promoter responsive to LldR and luxR | BBa_K2500011 |
| AND gate C | Synthetic promoter responsive to LldR and luxR | BBa_K2500012 |
| pConst_RBS_LldP/ LldR_pConst_RBS_LuxR |
Expression cassette consisting of LuxR and LldP/LldR | BBa_K2500013 |
Composite Parts
| Part | Description | BioBrick |
|---|---|---|
| Best Composite Part: pConst_RBSeng_TlpA |
Temperature-dependent transcriptional repressor TlpA regulated by constant promoter and engineered RBS | BBa_K2500008 |
Design
BBa_K2500010: AND gate a
In absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the binding sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcription. In design a, the distances between the intercalated promoter and the binding sites were adapted from BBa_K1847007.
Registry: BBa_K2500010
BBa_K2500011: AND gate b
In design b, each binding site was duplicated in order to achieve a potential zipper mechanism and stronger inhibition due to binding more LldR inhibitor proteins.
Registry: BBa_K2500011
BBa_K2500012: AND gate c
In design c, an artificial spacer was embedded between the pLux promoter and the O2 binding site in order to influence the looping dynamics.
Registry: BBa_K2500012
