Team:ETH Zurich/Parts
Parts
Overview
We submitted in total 14 new BioBricks to the iGEM registry! You can find all parts and their design below.
Basic Parts
| Part | Description | BioBrick |
|---|---|---|
| Bacterioferritin | Heme-deletion mutant of bacterial iron storage protein functioning as an MRI contrast agent | BBa_K2500000 |
| Azurin | Redox protein originating from P. aeruginosa with anti-cancer activity | BBa_K2500001 |
| p28 | Effector domain of azurin | BBa_K2500002 |
| pTlpA | Temperature-responsive promoter optimized for slight activation above 37°C and full activation at 45 °C | BBa_K2500003 |
| TlpA | Temperature-dependent transcriptional repressor of pTlpa | BBa_K2500004 |
| RBS_TlpA | Temperature-dependent transcriptional repressor of pTlpa and synthetic RBS | BBa_K2500005 |
| Protein E | Bacteria-lysing protein encoded by phage Phi X 17 | BBa_K2500006 |
| RBSeng_TlpA | Temperature-dependent transcriptional repressor of pTlpa with engineered RBS | BBa_K2500007 |
| RBSeng_ProteinE | Bacteria-lysing protein encoded by phage Phi X 174 with engineered RBS | BBa_K2500009 |
| AND Gate A | Synthetic promoter responsive to LldR and luxR | BBa_K2500010 |
| Best Basic Part: AND Gate B |
Synthetic promoter responsive to LldR and luxR | BBa_K2500011 |
| AND Gate C | Synthetic promoter responsive to LldR and luxR | BBa_K2500012 |
| pConst_RBS_LldP/ LldR_pConst_RBS_LuxR |
Expression cassette consisting of LuxR and LldP/LldR | BBa_K2500013 |
Composite Parts
| Part | Description | BioBrick |
|---|---|---|
| Best Composite Part: pConstRBSeng_TlpA |
Constitutively expressed temperature-dependent transcriptional repressor of pTlpa with engineered RBS | BBa_K2500008 |
Design
BBa_K2500010: AND gate A
In the absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the binding sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcription. In design A, the distances between the intercalated promoter and the binding sites were adapted from BBa_K1847007.
Registry: BBa_K2500010
BBa_K2500011: AND Gate B
In design B, each binding site was duplicated in order to achieve a potential zipper mechanism and stronger inhibition due to binding more LldR inhibitor proteins.
Registry: BBa_K2500011
BBa_K2500012: AND Gate C
In design C, an artificial spacer was embedded between the pLux promoter and the O2 binding site in order to influence the looping dynamics.
Registry: BBa_K2500012
