Team:Oxford/Protocols
Routine Cloning
We wanted to have a page of our wiki where we summarised the steps that we took from designing our parts through to sending them to iGEM to add to the registry, it is the sort of page we would have found useful when we first started so we had a good overview.
Create DNA in Snapgene
Create DNA in Snapgene
Order DNA from IDT
Order DNA from IDT
PCR from G-Blocks
PCR from G-Blocks
Run a Gel of the PCR
Run a Gel of the PCR
Digest and Ligate
Transform and Plate
Transform and Plate
Inoculate - Placeholder Image
Inoculate
Miniprep
Miniprep
Test Digest and Sequence
Test Digest and Sequence
Send DNA to iGEM
Send DNA to iGEM
