Basic Parts used in our Project
Our team used several basic parts to create our gene constructs. Here is an overview of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.
| Registry link |
Part |
Plasmid |
Summary |
Length |
| BBa_K2404000 |
TSH |
pSB1C3 |
A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification |
780bp |
| BBa_K2404001 |
TSHH |
pSB1C3 |
A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification |
832bp |
| BBa_K2404002 |
PDF1.2 |
pSB1C3 |
An inducible promoter that responds to the presence of jasmonic acid in the cell |
522bp |
| BBa_K2404003 |
PR2 |
pSB1C3 |
An inducible promoter that responds to the presence of salicylic acid in the cell |
519bp |
| BBa_K2404004 |
GST6 |
pSB1C3 |
An inducible promoter that responds to the presence of salicylic acid in the cell |
519bp |
| BBa_K2404005 |
WRKY30 |
pSB1C3 |
An inducible promoter that responds to the presence of cellulose-derived oligomers
After submission to the registry we discovered that this promotor sequence was incorrect |
507bp |
| BBa_K2404006 |
Luc+ |
pSB1C3 |
A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate, luciferin, which can be used to quantify gene expression. Prior to submission to the registry we discovered that this promotor sequence was incorrect so it was not added. In order to generate the level 1 clones containing LUC+ (BBa_K2404013, BBa_K240414) we used a previously generated LUC+ that is contained within a different (not pSB1C3) level 0 plasmid |
1653bp |
| BBa_P10401 |
NosT |
pSB1C3 |
A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37 |
285bp |
| BBa_P10000 |
LexA |
pSB1C3 |
An estradiol inducible promoter from the LexA gene which is native to Escherichia coli |
362bp |
| BBa_P10003 |
35S-OTMV |
pSB1C3 |
A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus |
1446bp |
