Team:SDU CHINA/basic parts

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Table of the Basic Parts we submitted to the BioBrick registry

Part Number	Name	Type	Length	Description
BBa_K2321000	hTERT promoter	Regulatory	809	tumor-specific promoter
BBa_K2321001	Survivin promoter	Regulatory	1024	tumor-specific promoter
BBa_K2321002	Luciferase	Reporter	1653	firefly luciferase reporter

Both hTERT and survivin are tumor-specific promoter, so the parameter of starting efficiency after transfection is most important. In order to explore the specificity and sensitivity of starting efficiency for each promoter, they were respectively inserted into double luciferase reporter vector. Then we selected two kinds of NSCLC cell lines, including A549， and measured the relative luciferase expression activity of the plasmids in different cell lines and different time points.

Table 1：Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on

TK plasmid in A549 cell line, after hTERT was inserted into pGL-3 vector.

Figure 1: The express efficiency of hTERT promoter in A549 cell line.

Table 2: Expression of fire fluorescense on pGL-3 plasmid and renilla fluorescense on

TK plasmid in A549 cell line, after survivin was inserted into pGL-3 vector.

Figure 2 :The express efficiency of survivin promoter in A549 cell line.

For more details, please refer to the results.