Team:ETH Zurich/Protocols

• Project Idea
  • Story of CATE
  • CATE in Action
  • Circuit
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  • Overview
  • Tumor Sensor
  • Heat Sensor
  • 3D Model
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Protocols

General Protocols

Chemical Transformation

Colony PCR

Consumables

Cryostock of Bacteria

Electroporation

Gel Extraction

Gibson Assembly

Ligase Chain Reaction

Miniprep

PCR Clean-Up

Phusion PCR

Preparation of Chemically Competent Cells

Preparation of Electrocompetent Cells

Restriction Ligation

Tumor Sensor

Quorum Sensing End-Point Characterization

AND-gate Without Quorum Sensing

AND-gate With Quorum Sensing

MRI Contrast Agent

Fluorescence Measurement to Obtain the AHL Dose-Response Curve

SDS-PAGE to Confirm AHL-Induced Expression of Bacterioferritin

Magnetic Resonance Imagine of Bacterioferritin-Expressing E. coli Nissle

Anti-Cancer Toxin

Fluorescence Measurement to Obtain the AHL Dose-Response Curve

SDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28

Heat Sensor

Heat-Induction of TlpA-Repressed GFP Expression

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