Team:Exeter/InterLab

In 2017, the iGEM InterLab Study asked teams to help developing a standardised curve of fluorescence measurements and to measure the relative strength of some unknown devices with different RBSs:

  • Device 1: BBa_J364000
  • Device 2: BBa_J364001
  • Device 3: BBa_J364002
  • Device 4: BBa_J364003
  • Device 5: BBa_J364004
  • Device 6: BBa_J364005
  • Positive Control: BBa_ I20270
  • Negative Control: BBa_R0040

This involved measuring the fluorescence and OD600 of cultures of E. coli DH5α transformed with each construct to compare the relative expression per cell. By asking all iGEM teams to use the same calibration process for the instruments used for the measurements and comparing the results to a measured standard curve, the relative units of fluorescence can be converted to absolute units. We started our contribution by resuspending the appropriate DNA from the iGEM distribution kit.

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