Team:BIT/MO2
Abstracts:
One part of our aim of modeling is to verify the effect of signal molecules on the promoter and to make the biological expression process clearer and more organized. What’s more, the main purpose is to verify the feasibility of the experiments of expression group and guide the progress of the experiment more smooth. However, the expression of the gene line is relatively long, if the theory is wrong, and the mistakes are found after the experiment, there will be a great harm on the experiment schedule, and moreover, the change will be very difficult. Therefore, the feasibility of modeling validation before expression experiments can provide a theoretical basis for expression groups and provide guidance for the success of their subsequent experiments. In addition, the detection of biomolecules is usually difficult to achieve, because the amount of biomolecules in the human body is too small, so we considered using the double fluorescence system to achieve the amplification by mathematical model and detect the amount of material on . Therefore, we use the support of literature , use the model to verify the linear relationship between the GFP and RFP ratio, to verify that the dual fluorescence system can be used for our project detection link, which play a guiding experimental team successfully completed the role of the experiment.
Objective:
One part of our aim of modeling is to verify the effect of signal molecules on the promoter and to make the biological expression process clearer and more organized. What’s more, the main purpose is to verify the feasibility of the experiments of expression group and guide the progress of the experiment more smooth. However, the expression of the gene line is relatively long, if the theory is wrong, and the mistakes are found after the experiment, there will be a great harm on the experiment schedule, and moreover, the change will be very difficult. Therefore, the feasibility of modeling validation before expression experiments can provide a theoretical basis for expression groups and provide guidance for the success of their subsequent experiments. In addition, the detection of biomolecules is usually difficult to achieve, because the amount of biomolecules in the human body is too small, so we considered using the double fluorescence system to achieve the amplification by mathematical model and detect the amount of material on . Therefore, we use the support of literature , use the model to verify the linear relationship between the GFP and RFP ratio, to verify that the dual fluorescence system can be used for our project detection link, which play a guiding experimental team successfully completed the role of the experiment.
Introduction:
In this model, we made the following assumptions: (1) having constructed lysine-deficient Escherichia coli and obtained accurate experimental data (2) the promoter sequence unchanged after the inhibition of protein binding to the promoter. (3) the terminator can completely terminate the promoter (4) When the inhibitory protein is absent, the promoter can stably express red fluorescent protein (RFP) (5) don’t consider the expression of the leakage of gene lines.
The first part: The gene line which produce green fluorescent
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