Our constructs
Chaperone systems
We designed one (super)plasmid that containd the genes for the three different cheperone systems we are useing. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone PSB1C3 for our (super)plasmid.
Amyloid-beta
We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to M-neongreen protein.