Team:Linkoping Sweden/construct
Our constructs
Chaperone systems
We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroEs, DnaK and trigger factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone PSB1C3 for our superplasmid.
Amyloid-beta
We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to M-neongreen protein. For these plasmids we used an arabinose promotor. We put these substrates in the PSB1A3 backbone from iGEM.
Tau
We designed one plasmid with Tau bound to GFP and one plasmid with Tau bound to M-neongeen protein. We used the arabinose promotor here.
