Team:ETH Zurich/Design
Design
Overview
On this page we explain the design principles we defined for our project and how we followed them. We structured our work in phases and tried to proceed through them. The phases apply to theoretical work (models) as well as to the practical (experiments). In the early phases, we learned to handle the subjects and get familiar with the theory and literature. We designed, ordered and built constructs for tests of the experimental procedure and for further optimization. In later phases, we tested predictions of the models and delivered parameters for new models. We engineered single parts to behave as our circuit requires. This project was designed in a hierarchical bottom-up engineering approach: The individual parts were constructed with molecular cloning and their functions were tested with reporter genes.
We created reduced libraries (Red Libs) to create variants with an expression level that fits the needs of the circuit. As soon as the parts met our requirements, we combined them to higher level contructs and tested them again for the combined function.
Experiments Plan
Here you find the overview of the phases of our project and which parts were used at which time.
Experiments Plan
Here you find the overview of the phases of our project and which parts were used at which time.
