Interlab
iGEM's interlab study aims to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. This means teams have to use the same exact protocol around the world to produce common, comparable units for measuring GFP with different plate readers. For more information on the protocol, check out iGEM's official Interlab Study webpage. Below is the data that we collected for the Interlab Study:
OD600 Reference Point |
Plate PatternThis diagram depicts how our samples were organized on our plate: |
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uM Fluorescein/a.u.
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Curves
Abs600 Raw Readings
These tables summarize our finalized sets of Absorbance reading data over an incubation period of 6 hours with samples measured every 2 hours for a total of 4 time points:
Hour 0
Hour 0:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
0.0935
0.0951
0.0942
0.0917
0.0918
0.0925
0.0919
0.0933
0.0902
C1, R2
0.0937
0.0937
0.0973
0.0941
0.0937
0.096
0.0937
0.0945
0.0886
C1, R3
0.0946
0.0939
0.1013
0.0957
0.0931
0.1026
0.1074
0.0945
0.0904
C1, R4
0.0982
0.0943
0.0996
0.0942
0.0926
0.0939
0.094
0.0953
0.0899
C2, R1
0.0932
0.0944 0.0956 0.0933 0.0927 0.0919 0.0934 .0973
0.0894
C2, R2
0.0928 0.0949 0.0975 0.0967 0.0976
0.0949 0.0962 0.0957 0.0894
C2, R3
0.0925 0.094 0.0983 0.095 0.0939 0.0941 0.0946
0.0955 0.0916
C2, R4
0.0918 0.0965 0.0958 0.0942
0.0939 0.0923 0.093 0.0932 0.0887
Hour 2
Hour 2:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
0.1289 0.1165 0.0932 0.1002 0.1056 0.0952
0.1129 0.1145 0.0892
C1, R2
0.1294 0.1158 0.0975 0.1031 0.1087
0.0973 0.1136 0.117 0.0922
C1, R3
0.1305 0.1156 0.0962 0.1013 0.108 0.0982
0.1157 0.1174 0.0916
C1, R4
0.1275 0.1153 0.096 0.1019 0.11 0.0964
0.1115 0.1189 0.0845
C2, R1
0.1273 0.1221 0.0957 0.1027 0.1165 0.0956
0.1147 0.1118 0.0844
C2, R2
0.129 0.1192 0.0958 0.1033 0.1198 0.0935
0.1162 0.114 0.0922
C2, R3
0.1246 0.1221 0.0961 0.1021 0.1178 0.096
0.1156 0.1114 0.0921
C2, R4
0.1258 0.1227 0.0969 0.0995 0.121 0.096
0.1139 0.1134 0.0895
Hour 4
Hour 4:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
0.1906 0.1708 0.0939 0.1434
0.1767 0.1029 0.1698 0.1784 0.0908
C1, R2
0.1923 0.1717 0.0953 0.1479 0.179 0.1045
0.1761 0.1805 0.0904
C1, R3
0.1951 0.1747 0.1072 0.1504 0.1809 0.1049
0.1741 0.184 0.0911
C1, R4
0.1996 0.1797 0.0979 0.1519 0.1833 0.109
0.1788 0.1824 0.0906
C2, R1
0.1889 0.1833 0.0961 0.1369
0.1802 0.0958 0.1703 0.1872 0.0902
C2, R2
0.1903 0.1844 0.0986 0.1368 0.1863 0.0988
0.171 0.1956 0.092
C2, R3
0.189 0.1891 0.0971 0.139 0.1821 0.0974
0.1701 0.1874 0.0958
C2, R4
0.1891 0.1865 0.0953 0.1417 0.1815 0.0976
0.167 0.1845 0.0895
Hour 6
Hour 6:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
0.2852 0.2515 0.0937 0.2081 0.2876
0.1312 0.2516 0.2616 0.0902
C1, R2
0.2722 0.2545 0.0956 0.2188 0.2918
0.1308 0.2483 0.2663 0.0907
C1, R3
0.2719 0.2535 0.0956 0.2184 0.2929 0.1328
0.2542 0.2714 0.0912
C1, R4
0.2815 0.2591 0.0971 0.2148 0.2959
0.1347 0.2488 0.2649 0.0905
C2, R1
0.2634 0.2673 0.0954 0.1858 0.2583
0.102 0.2342 0.2635 0.0901
C2, R2
0.2651 0.2679 0.097 0.1857 0.2638 0.1042
0.2372 0.2719 0.0926
C2, R3
0.268 0.2681 0.0952 0.1875 0.2624
0.1047 0.2326 0.2628 0.0928
C2, R4
0.2525 0.2702 0.1024 0.1837 0.2582 0.1034
0.2384 0.2648 0.0901
Fluorescence Raw Readings
These tables summarize our finalized sets of Fluorescent reading data over an incubation period of 6 hours:
Hour 0
Hour 0:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
63 69 130 83 64 76 66
64 64
C1, R2
63 72 129 84 64
75 70 65 64
C1, R3
64 70 131 84 65 76
66 66 67
C1, R4
64 72 132 84 65
77 67 65 65
C2, R1
63 71 124 81 63 74
68 65 66
C2, R2
63 71 122 82 63
74 65 65 68
C2, R3
64 71 123 82 64
75 67 63 67
C2, R4
64 71 123 83
64 75 65 65 66
Hour 2
Hour 2:
Neg. Control
Pos. Control
Device 1
Device 2
Device 3
Device 4
Device 5
Device 6
LB + Chlor (blank)
C1, R1
67 112 135 135
70 97 94 65 65
C1, R2
68 113 139 134
71 98 96 67 67
C1, R3
66 113 142 137 69
97 98 68 66
C1, R4
66 115 136 139 71
95 95 65 70
C2, R1
65 122 133 128
67 86 95 66 70
C2, R2
64 121 136 129 66
84 97 65 65
C2, R3
63 118 133 131
66 86 97 65 66
C2, R4
63 117 136 129
67 86 98 64 65
Abs600 Raw Readings
These tables summarize our finalized sets of Absorbance reading data over an incubation period of 6 hours with samples measured every 2 hours for a total of 4 time points:
Hour 0
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.0935 | 0.0951 | 0.0942 | 0.0917 | 0.0918 | 0.0925 | 0.0919 | 0.0933 | 0.0902 |
C1, R2 | 0.0937 | 0.0937 | 0.0973 | 0.0941 | 0.0937 | 0.096 | 0.0937 | 0.0945 | 0.0886 |
C1, R3 | 0.0946 | 0.0939 | 0.1013 | 0.0957 | 0.0931 | 0.1026 | 0.1074 | 0.0945 | 0.0904 |
C1, R4 | 0.0982 | 0.0943 | 0.0996 | 0.0942 | 0.0926 | 0.0939 | 0.094 | 0.0953 | 0.0899 |
C2, R1 | 0.0932 | 0.0944 | 0.0956 | 0.0933 | 0.0927 | 0.0919 | 0.0934 | .0973 | 0.0894 |
C2, R2 | 0.0928 | 0.0949 | 0.0975 | 0.0967 | 0.0976 | 0.0949 | 0.0962 | 0.0957 | 0.0894 |
C2, R3 | 0.0925 | 0.094 | 0.0983 | 0.095 | 0.0939 | 0.0941 | 0.0946 | 0.0955 | 0.0916 |
C2, R4 | 0.0918 | 0.0965 | 0.0958 | 0.0942 | 0.0939 | 0.0923 | 0.093 | 0.0932 | 0.0887 |
Hour 2
Hour 2: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.1289 | 0.1165 | 0.0932 | 0.1002 | 0.1056 | 0.0952 | 0.1129 | 0.1145 | 0.0892 |
C1, R2 | 0.1294 | 0.1158 | 0.0975 | 0.1031 | 0.1087 | 0.0973 | 0.1136 | 0.117 | 0.0922 |
C1, R3 | 0.1305 | 0.1156 | 0.0962 | 0.1013 | 0.108 | 0.0982 | 0.1157 | 0.1174 | 0.0916 |
C1, R4 | 0.1275 | 0.1153 | 0.096 | 0.1019 | 0.11 | 0.0964 | 0.1115 | 0.1189 | 0.0845 |
C2, R1 | 0.1273 | 0.1221 | 0.0957 | 0.1027 | 0.1165 | 0.0956 | 0.1147 | 0.1118 | 0.0844 |
C2, R2 | 0.129 | 0.1192 | 0.0958 | 0.1033 | 0.1198 | 0.0935 | 0.1162 | 0.114 | 0.0922 |
C2, R3 | 0.1246 | 0.1221 | 0.0961 | 0.1021 | 0.1178 | 0.096 | 0.1156 | 0.1114 | 0.0921 |
C2, R4 | 0.1258 | 0.1227 | 0.0969 | 0.0995 | 0.121 | 0.096 | 0.1139 | 0.1134 | 0.0895 |
Hour 4
Hour 4: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.1906 | 0.1708 | 0.0939 | 0.1434 | 0.1767 | 0.1029 | 0.1698 | 0.1784 | 0.0908 |
C1, R2 | 0.1923 | 0.1717 | 0.0953 | 0.1479 | 0.179 | 0.1045 | 0.1761 | 0.1805 | 0.0904 |
C1, R3 | 0.1951 | 0.1747 | 0.1072 | 0.1504 | 0.1809 | 0.1049 | 0.1741 | 0.184 | 0.0911 |
C1, R4 | 0.1996 | 0.1797 | 0.0979 | 0.1519 | 0.1833 | 0.109 | 0.1788 | 0.1824 | 0.0906 |
C2, R1 | 0.1889 | 0.1833 | 0.0961 | 0.1369 | 0.1802 | 0.0958 | 0.1703 | 0.1872 | 0.0902 |
C2, R2 | 0.1903 | 0.1844 | 0.0986 | 0.1368 | 0.1863 | 0.0988 | 0.171 | 0.1956 | 0.092 |
C2, R3 | 0.189 | 0.1891 | 0.0971 | 0.139 | 0.1821 | 0.0974 | 0.1701 | 0.1874 | 0.0958 |
C2, R4 | 0.1891 | 0.1865 | 0.0953 | 0.1417 | 0.1815 | 0.0976 | 0.167 | 0.1845 | 0.0895 |
Hour 6
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 0.2852 | 0.2515 | 0.0937 | 0.2081 | 0.2876 | 0.1312 | 0.2516 | 0.2616 | 0.0902 |
C1, R2 | 0.2722 | 0.2545 | 0.0956 | 0.2188 | 0.2918 | 0.1308 | 0.2483 | 0.2663 | 0.0907 |
C1, R3 | 0.2719 | 0.2535 | 0.0956 | 0.2184 | 0.2929 | 0.1328 | 0.2542 | 0.2714 | 0.0912 |
C1, R4 | 0.2815 | 0.2591 | 0.0971 | 0.2148 | 0.2959 | 0.1347 | 0.2488 | 0.2649 | 0.0905 |
C2, R1 | 0.2634 | 0.2673 | 0.0954 | 0.1858 | 0.2583 | 0.102 | 0.2342 | 0.2635 | 0.0901 |
C2, R2 | 0.2651 | 0.2679 | 0.097 | 0.1857 | 0.2638 | 0.1042 | 0.2372 | 0.2719 | 0.0926 |
C2, R3 | 0.268 | 0.2681 | 0.0952 | 0.1875 | 0.2624 | 0.1047 | 0.2326 | 0.2628 | 0.0928 |
C2, R4 | 0.2525 | 0.2702 | 0.1024 | 0.1837 | 0.2582 | 0.1034 | 0.2384 | 0.2648 | 0.0901 |
Fluorescence Raw Readings
These tables summarize our finalized sets of Fluorescent reading data over an incubation period of 6 hours:
Hour 0
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 63 | 69 | 130 | 83 | 64 | 76 | 66 | 64 | 64 |
C1, R2 | 63 | 72 | 129 | 84 | 64 | 75 | 70 | 65 | 64 |
C1, R3 | 64 | 70 | 131 | 84 | 65 | 76 | 66 | 66 | 67 |
C1, R4 | 64 | 72 | 132 | 84 | 65 | 77 | 67 | 65 | 65 |
C2, R1 | 63 | 71 | 124 | 81 | 63 | 74 | 68 | 65 | 66 |
C2, R2 | 63 | 71 | 122 | 82 | 63 | 74 | 65 | 65 | 68 |
C2, R3 | 64 | 71 | 123 | 82 | 64 | 75 | 67 | 63 | 67 |
C2, R4 | 64 | 71 | 123 | 83 | 64 | 75 | 65 | 65 | 66 |
Hour 2
Hour 2: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
C1, R1 | 67 | 112 | 135 | 135 | 70 | 97 | 94 | 65 | 65 |
C1, R2 | 68 | 113 | 139 | 134 | 71 | 98 | 96 | 67 | 67 |
C1, R3 | 66 | 113 | 142 | 137 | 69 | 97 | 98 | 68 | 66 |
C1, R4 | 66 | 115 | 136 | 139 | 71 | 95 | 95 | 65 | 70 |
C2, R1 | 65 | 122 | 133 | 128 | 67 | 86 | 95 | 66 | 70 |
C2, R2 | 64 | 121 | 136 | 129 | 66 | 84 | 97 | 65 | 65 |
C2, R3 | 63 | 118 | 133 | 131 | 66 | 86 | 97 | 65 | 66 |
C2, R4 | 63 | 117 | 136 | 129 | 67 | 86 | 98 | 64 | 65 |