Team:ICT-Mumbai/Safety
The host organism of our work is the ‘lab pet’ / ‘industrial workhorse’ Escherichia coli. We have used different strains of E. coli for our work – DH5α, BW25113, BL21(DE3) – all of which are categorised under Biosafety Level I (BSL I) and safe to use and handle.
We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal.