Team:Jilin China/Basic Part
wt-Pr is a constitutive promoter which is in the upstream of DmpR, a transcriptional factor of dmp operon from Pseudomonas sp. Strain CF600 encoded by dmpR gene[1].
2.cphA-1
CphA-1, a catechol 1,2-dioxygenase from Arthrobacter chlorophenolicus A6, is responsible for ring cleavage in aromatic compounds degrading process [2].
Figure 2. Reaction of cphA-1
3.CaO19
CaO19, a hydroxyquinol 1,2-dioxygenase from Candida albicans TL3, is responsible for ring cleavage in aromatic compounds degrading process[3].
Figure 3. Reaction of CaO19
4.CbtA
CbtA is a protein found in crytic prophage CP4-44 in Escherichia coli K-12. CbtA could inhibit cell division and cell elongation via direct and independent interactions with FtsZ and MreB[4], so it is defined as a kind of toxin.
5.CbeA
CbeA is a protein found in crytic prophage CP4-44 in Escherichia coli K-12 which could suppress the effect of CbtA, so it is defined as a kind of antitoxin. Instead of interacting with CbtA, CbeA directly binds MreB and FtsZ and promotes the assembly of FtsZ and MreB filaments[4].
Figure 4. TA system
This year we use CbtA and CbeA to build the Geneguard system in our project. For detailed information about toxin/antitoxin (TA) system, please visit ...
Reference：[1] Shingler, V., M. Bartilson, and T. Moore. Cloning and nucleotide sequence of the gene encoding the positive regulator (DmpR) of the phenol catabolic pathway encoded by pVI150 and identification of DmpR as a member of the NtrC family of transcriptional activators. J. Bacteriol. ( 1993) 175: 1596–1604.
[2] Seok H. Lee. Effective biochemical decomposition of chlorinated aromatic hydrocarbons with a biocatalyst immobilized on a natural enzyme support. Bioresource Technology. (2013) 141:89–96.
[3] Purification and characterization of a catechol 1,2-dioxygenase from a phenol degrading Candida albicans TL3. San-Chin Tsai · Yaw-Kuen Li Arch Microbiol. (2007) 187:199–206.
[4] Masuda, Tan. YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli. Molecular Microbiology. (2012) 84(5), 979–989.
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