Team:NUS Singapore/Future Work
Future work
Currently we have only characterized four different sensors as a proof of concept for our sensor library. In the future, we will be expanding the sensor library by characterizing more sensors into our toolkit so that we can develop this toolkit as a platform technology to make engineering kill switches much easier. Moreover, the current experiments focused on E.coli as a model organism and we will be expanding our characterization into Gram positive bacteria such as Lactobacillus which are used more frequently in engineered probiotics. In terms of modelling, we will be testing more modeling tools to figure out the optimal ways to construct genetic circuits for more effective killing. Since the existing models provided by CELLO usually have high metabolic stress and longer response time, we will also be exploring the possibility of simplifying the genetic circuit via a software to circumvent such shortcomings of a CELLO system.
