Red - working on RF cloning
Green - working on new genes
Blue - working on yeast cells
Orange - working on Brett
Purple - working on improve BioBrick
27.8.17
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S.cerevisiae 3095 with URA- mutation were obtained from Dr. Itay Onn (faculty of medicine of Bar Ilan University) and were isolated from YEPD plates.
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Isolation streak of Brett on selection medium- specific YEPD media.
29.8.17
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Colonies from Brett isolation appeared.
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Colonies that appeared were tested using PCR with specific primers (from Prof. Martin Goldway lab) for Brett detection- PB2, RUX, DB, and NLS. We got negative results- no PCR product.
Fig.1- Dana having fun with Brett colonies in the lab
