Red - working on RF cloning
Green - working on new genes
Blue - working on yeast cells
Orange - working on Brett
Purple - working on improve BioBrick
27.8.17
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S.cerevisiae 3095 with URA- mutation were obtained from Dr. Itay Onn (faculty of medicine of Bar Ilan University) and were isolated from YEPD plates.
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Isolation streak of Brett on selection medium- specific YEPD media.
29.8.17
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Colonies from Brett isolation appeared.
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Colonies that appeared were tested using PCR with specific primers (from Prof. Martin Goldway lab) for Brett detection- PB2, RUX, DB, and NLS. We got negative results- no PCR product.
Fig.1- Dana having fun with Brett colonies in the lab
30.8.17
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We got pRS306/316 from Dr. Itay Onn.
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Transformation into TOP-10 competent cells:
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Preparation of competent TOP10 e.coli bacteria for heat-shock transformation (we used this protocol – לתת לינק לדף פרוטוקול מספר 9)
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T--Tel-Hai--dana-in-the-labTransformation for plasmids pRS306 + pRS316 into competent cells was performed using Heat-shock protocol then incubated O.N.
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