Attributions
In the course of our project we were able to conduct experiments never done before. However, in order to do so a base of knowledge and resources is required. Fortunately we encountered many ambitious people who supported us. We want to thank them all for kindly helping us during our project.
- • Gabi Wilt: ordered Materials for our Team
- • Corina Rebnegger: helped us doing Flow cytometric experiments for characterization of our pGAL syn
- • Tom Gassler: gived us advice for setting up our CRISPR Cas9 mediated gene integration in Saccharomyces cerevisiae by supporting us with primers for assembling the specific sgRNAs.
- • Martin Altvater: provided us different plasmids for Saccharomyces cerevisiae ready for our Golden Gate system
- • Jürgen Zanghelini: supported us with our modelling approaches
- • Florian Strobl:
- • Gerald Striedner and Esther Egger provided us the plasmids pSIM 5 and pSIM 9.
- • Brigitte Lang
- • Matthias Steiger
- • Franziska Doleschal: organized our bank account and managed all our invoices.
- • Anna Gindl: designed our Logo
- • Nikolaus Huber: helped us setting up the core code of our Wiki page
- • AG Sauer: Provided us BB1_01 – BB1_08
- • AG Mattanovich/Gasser: provided us material, all empty BBs for the Golden GATE system and many protocols:
- • Preparation of chemical competent E. coli
- • Transformation chemical competent E. coli
- • Preparation of electro competent S. cerevisiae
- • Transformation of electro competent S. cerevisiae
- • mRNA extraction
- • Bernd Albrecht: helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters.