Team:Harvard/Basic Parts
Parts
This year we submitted 5 new parts to the parts registry.
The parts feature varying strength RBS in front of csgG, the porous protein involved in the secretion of monomeric csgA in wild-type E. coli. This part can be stitched together with a standard promoter and compared with other similar parts with different RBSs to see some varying level of csgG expression.
Origins of Sequences
The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the Salis Lab RBS generator .Design
The RBS sequence was generated from a randomized library of RBSs using the Salis Lab RBS generator. Then, plasmids with desired RBS strengths were screened for and selected. Refer to our protocol page for details in procedure.
