Team:IIT Delhi/recipe

iGEM IIT Delhi

RECIPES

Accurate Experimenting for Accurate Results !!

20x TAE buffer

80mL of 1M Tris solution
0.7264g of EDTANa2 salt
2.284 mL glacial acetic acid
distilled H2O 100mL

For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.

50 mM glucose.
25 mM Tris-Cl (pH 8.0).
10 mM EDTA (pH 8.0).

Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by autoclaving and store at 4°C.

0.2 N NaOH (freshly diluted from a 10 N stock).(100ulsds + 700ul mq + 200ul naoh) 1% (w/v) SDS.

5 M potassium acetate, 60.0 ml
Glacial acetic acid, 11.5 ml
H2O, 28.5 ml The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate. Store the solution at 4°C and transfer it to an ice bucket just before use.

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	E-mail: iitd.igem@gmail.com Undergraduate Laboratory Department of Biotechnology and Biochemical Engineering, IIT Delhi