Results

Metagenomic Library

DNA Extraction

Over the course of the 2017 iGEM season, we have had some downs, but many more ups.

Successes

Developed a pipeline to identify, or "mine", the porcupine metagenomic sequencing to discover novel enzymes.
Identified 8? novel enzymes with variable percent identity.
Synthesized 5 of those enzymes, and successfully cloned 4 of them into psB1AK3.
Optimized our previous biobrick Endoglucanse(BBa_K2160000)by adding a C termincal HIS-tag and N terminal PelB sequence (Improve).
Successfully completed a fluorophore cleavage assay from the Hallam lab.
Isolated high molecular weight DNA from porcupine fecal samples.
Obtained efficient ligation and digestion with pJC8 controls.
Produced phage plaque with the phage packaging extract lamba DNA controls.

Failures

Did not clone our biobricks into the shipping vector psB1C3
Was not able to achieve the right environment for our novel beta-xylanase to function
Was not able to design a functional media assay for our enzymes
Could not make a functional metagenomic library
Sheered our high molecular weight DNA

Team:Dalhousie/Results